The Hemostasis and Thrombosis Research Laboratories within the Department of Pathology represent a multidisciplinary group of laboratories dedicated to the investigation on the mediators of the pathogenesis of vascular, and cardiovascular disorders. The laboratories are also involved in the study of new drugs to treat thrombotic and bleeding disorders. Dr. Fareed's research work on the pathogenesis of thrombotic disorders focuses on the role of plasmatic, vascular, and cellular mediators of thrombogenesis, in particular the role of inflammation in thrombotic processes.
Jawed Fareed, PhD
- Professor, Departments of Pathology and Pharmacology
- Director of the Special Coagulation Laboratory and the Hemostasis and Thrombosis Research Program
The areas of interest are outlined below
Pathogenesis of sepsis assoiaited coagulopathies
An integrated program to understand the markers of the pathogenesis of sepsis associated coagulopathy with respect to DIC is developed. Biomarker identification and characterization and proteomic approaches are utilized.
Relevance of the circulating levels of tissue factor and related mediators to vascular and thrombotic disorders
Specific methods to measure the circulating levels of tissue factor are developed and validated in patient groups such as atherosclerosis, hypertension, acute coronary syndromes, transplant-associated vascularopathies, sickle cell anemia, bone marrow transplantation-associated VOD, and cancer. Tissue factor levels have been measured in these groups.
Tissue factor pathway inhibitor (TFPI) and its regulation in various pathologic conditions.
Several functional and immunologic methods to quantitated TFPI in biological fluids have been measured in various pathologic states. In addition, the effects of such drugs as heparin and defibrotide are also measured. The objective of this study is to develop the prognostic role of TFPI in various diseases. Recombinant forms of TFPI and its variants are also used to study the interactions of TFPI to various enzymes and TF.
Role of adhesion molecules in thrombotic disorders.
Using immunologic and flow cytometric methods, the role of circulating adhesion molecules such as ICAM, VCAM, P, E, and L selectins is investigated in arterial and venous thrombosis. In particular, the role of markers in thrombocytopenia and arterial thrombosis are investigated. Specific agents to control the generation of the adhesion molecules are also investigated.
Pathogenesis of end stage renal disease
An integrated program on the understanding of the pathogenesis of end stage renal disease is ongoing with reference to the role of newer mediators of inflammation.
Studies on the measurement of antiplatelet and antiprotease actions of low molecular weight heparins
Low molecular weight heparins have now become the gold standard in the prophylaxis and treatment of deep vein thrombosis and pulmonary embolism. Dr. Fareed's laboratories have been engaged in the study of the action of these agents and laboratory monitoring for nearly 20 years. Specific assay methods to monitor these drugs have been developed. Several animal models of thrombosis have been developed to simulate venous and arterial thrombosis. These models provide useful means to simulate thrombotic events. These models have also been used to compare the antiprotease and bleeding effects of these agents.
Immunogenic responses to therapeutic proteins
This program is formed to investigate the role of antibodies generated after therapeutic protein exposure in human and animal models.
Thrombogenesis and the mechanism of antithrombotic drugs in primates
Loyola University's division of Comparative Medicine in conjunction with the Department of Pathology maintains a primate colony to study the mechanism of thrombogenesis. The primate hemostatic proteins and cellular receptors (e.g., GP IIb/IIIa, GP Ib) exhibit a certain degree of homology to humans. Most of the antibodies raised against the coagulation proteins/receptors interact with primate blood proteins and cellular receptors. This model is used to simulate various thrombotic states (DVT, arterial thrombosis) and has been extremely useful in the study of tissue factor, plasma inhibitors, adhesion molecules, and other mediators. These primates are directly used in the development of pharmacokinetic and pharmacodynamic studies with drugs .
Animal models of vascular disorders
In conjunction with the comparative medicine faculty, several animal models to investigate bleeding and clotting disorders have been developed. In addition, hemodynamic disorders, cancer models and pharmacokinetic and pharmacodynamic parameter profiling in animal is also addressed.
The Hemostasis and Thrombosis Unit at Loyola University Medical Center has also developed a major core laboratory to validate the diagnostic value of newer markers of thrombogenesis. Global clot based, immunologic, platelet aggregation and activation assays; flow cytometric methods; and molecular analysis of coagulation defects are currently available. Core laboratory facilities to assist in large clinical trials (epidemiology) and drug evaluations are currently carried out in conjunction with various agencies. This laboratory is accredited by the joint commission, and all work is carried out under GLP guidelines.
View a partial list of Dr. Fareed's publications through the National Library of Medicine's PubMed online database.