Jawed Fareed, PhD Professor Departments of Pathology and Pharmacology Director of the Special Coagulation Laboratory and the Hemostasis and Thrombosis Research Program (708) 216-3262 JAFAREED@lumc.edu

The Hemostasis and Thrombosis Research Laboratories within the Department of Pathology represent a multidisciplinary group of laboratories dedicated to the diagnosis and management of thrombotic, vascular, and cardiovascular disorders. The laboratories are also involved in the study of new drugs. Dr. Fareed's research work on the pathogenesis of thrombotic disorders focuses on the role of plasmatic, vascular, and cellular mediators of thrombogenesis, in particular the study of tissue factor in the mediation of the thrombotic efficacy in such arterial diseases as thrombotic stroke and myocardial infarction.

The areas of interest are outlined below

Relevance of the circulating levels of tissue factor to vascular and thrombotic disorders.
Specific methods to measure the circulating levels of tissue factor are developed and validated in patient groups such as atherosclerosis, hypertension, acute coronary syndromes, transplant-associated vascularopathies, sickle cell anemia, bone marrow transplantation-associated VOD, and cancer. Tissue factor levels have been measured in these groups.

Tissue factor pathway inhibitor (TFPI) and its regulation in various pathologic conditions.
Several functional and immunologic methods to quantitated TFPI in biological fluids have been measured in various pathologic states. In addition, the effects of such drugs as heparin and defibrotide are also measured. The objective of this study is to develop the prognostic role of TFPI in various diseases. Recombinant forms of TFPI and its variants are also used to study the interactions of TFPI to various enzymes and TF.

Role of adhesion molecules in thrombotic disorders.
Using immunologic and flow cytometric methods, the role of circulating adhesion molecules such as ICAM, VCAM, P, E, and L selectins is investigated in arterial and venous thrombosis. In particular, the role of markers in thrombocytopenia and arterial thrombosis is investigated. Specific agents to control the generation of the adhesion molecules are also investigated.

Studies on the measurement of antiplatelet and antiprotease actions of low molecular weight heparins.
Low molecular weight heparins have now become the gold standard in the prophylaxis and treatment of deep vein thrombosis and pulmonary embolism. Dr. Fareed's laboratories have been engaged in the study of the action of these agents and laboratory monitoring for nearly 20 years. Specific assay methods to monitor these drugs have been developed. Several animal models of thrombosis have been developed to simulate venous and arterial thrombosis. These models provide useful means to simulate thrombotic events. These models have also been used to compare the antiprotease and bleeding effects of these agents.

Thrombogenesis and the mechanism of antithrombotic drugs in primates.
Loyola University's division of Comparative Medicine in conjunction with the Department of Pathology maintains a primate colony to study the mechanism of thrombogenesis. The primate hemostatic proteins and cellular receptors (e.g., GP IIb/IIIa, GP Ib) exhibit a certain degree of homology to humans. Most of the antibodies raised against the coagulation proteins/receptors interact with primate blood proteins and cellular receptors. This model is used to simulate various thrombotic states (DVT, arterial thrombosis) and has been extremely useful in the study of tissue factor, plasma inhibitors, adhesion molecules, and other mediators. These primates are directly used in the development of pharmacokinetic and pharmacodynamic studies with drugs .

The Hemostasis and Thrombosis Unit at Loyola University Medical Center has also developed a major core laboratory to validate the laboratory parameters of thrombotic defects. Global clot based, immunologic, platelet aggregation and activation assays; flow cytometric methods; and molecular analysis of coagulation defects are currently available. Core laboratory facilities to assist in large clinical trials (epidemiology) and drug evaluations are currently carried out in conjunction with various agencies. This laboratory is accredited by the joint commission, and all work is carried out under GLP guidelines.

Publications

View a partial list of Dr. Fareed's publications through the National Library of Medicine's PubMed online database.