|
Howard P. Greisler, M.D.
Professor,
Department of Surgery
Division
of Peripheral and Vascular Surgery
Joint
Professor,
Department
of Cell Biology, Neurobiology and Anatomy |
|
- B.A., Psychology, 1971. University of Pennsylvania, Philadelphia, PA
- M.D., Hershey Medical Center, 1975. Penn State University
- Fellowships, Vascular Surgery and Transplantation Surgery, 1980-1981.
Columbia-Presbyterian Medical Center, New York, N.Y.
|
Current Interests |
| Intimal hyperglasia, growth factors, tissue
engineering, smooth muscle cells, endothelial cells |
Laboratory Focus |
| Our laboratory focuses on the role
of growth factors as mediators of the vascular smooth muscle cell proliferation central to
atherogenesis and to restenosis following clinical interventions and uses principles of
tissue engineering to deliver SMC growth inhibitors and/or endothelial cell mitogens to
inhibit myointimal hyperplasia and to induce endothelialization. Our current ongoing
studies include local delivery systems for a battery of recombiant FGF mutants designed
for greater EC specificity, the development of novel bioresorbable polymers to induce
differential growth factor synthesis by phagocytizing macrophages, he delivery of
antisense oligonucleotides and of chimeric mitotoxins to selectively inhibit proliferating
SMC’s and the seeding of unmodified and genetically modified ECs onto both
de-endothelialized arteries and onto vascular grafts. Both cell culture and animal models
are used to test efficacy while cell responses are investigated by histologic, biochemical
and molecular analyses.
|
Publications |
| Kwon S, Xue L, Tassiopoulos AK, GREISLER HP:
Chemotaxis of Canine Endothelial Cells and Smooth Muscle Cells using an Under-Fibrin Glue
Assay, submitted to J Vasc Surg. 2001. |
Xue L, Shireman P, Woloson S, Tassiopoulos AK, Haudenschild C,
Kim DU, GREISLER HP: Fibrin hydrogel based VEGF delivery for acceleration of
arterial re-endothelialization, submitted to Journal of Surgical Research 2002. |
Xue L, GREISLER HP: Angiogenic effect of fibroblast
growth factor-1 and vascular endothelial growth factor and their synergism in a novel in
vitro quantitative fibrin-based three-dimensional angiogenesis system, Surgery
132(2); 259-267, 2002. |
GREISLER HP, Regulated in
vivo remodeling, Ann NY Acad Sci June; 961:309-11, 2002. |
| Erzurum V, Xue, L, Burgess W, GREISLER HP:
The R136K Fibroblast Growth Factor-1 Mutant Induces Heparin-Independent Migration of
Endothelial Cells Through Fibrin Glue. J Vasc Surg. , 37(5); 1075-1081, 2003. |
| Xue, Lian and GREISLER HP, Invited
Review: Biomaterials in the Development and future of Vascular Grafts, J Vasc Surg.;
37 (2), 472 – 481, 2003. |
| Laredo J, Xue L, Husak V, Ellinger J, GREISLER
HP: Silyl-heparin adsorption improves the in vivo thromboresistance of carbon-coated
polytetrafluoroethylene vascular grafts, Amer J Surgery ; 186 (5) 556-560, 2003. |
| Leon L, GREISLER HP: Vascular Grafts,
Expert Rev. Cardiovasc. Ther ; 1(14) 581-594, 2003. |
| Xue L, Sharma R, Cochran K, Tassiopoulos A,
Jara-Almonte J, Cromack K, Zage J, Wilding N, Tarcha P, GREISLER HP: The Effect of
the Rapamycin Derivative ABT-578 on Canine Smooth Muscle Cell and Endothelial Cell
Proliferation, Preclinica, , Nov-Dec, 2(6), 2004. |
| Laredo J, Xue L, Husak V, Ellinger J, Singh G,
Zamora P and GREISLER HP: Silyl-heparin bonding improves the patency and in vivo
thromboresistance of carbon-coated polytetrafluoroethylene vascular grafts. J Vasc Surg
; 39(5) 1059-65, 2004. |
| GREISLER HP: Induction of
Endothelialization of Tissue-Engineered Cardiovascular Constructs via In Vivo Angiogenesis
in Post Implant Remodeling. Atherosclerosis XIII. Volume 1262:115-117. May 2004. |
Brey E, Uriel S, GREISLER HP, Patrick C, McIntire L:
Therapeutic neovascularization: contributions from bioengineering. Tissue
Engineering. In Press. September 2004. |
| Brewster LP, Brey EM, Tassiopoulos AK, Xue L,
Maddox E, Armistead D, Burgess WH and GREISLER HP: Heparin-independent mitogenicity
in and endothelial and smooth muscle cell chimeric growth factor (S130K-HBGAM) Amer
Jour Surg ; 188 (5) 575-579, 2004. |
Brewster LP, Brey EM,
Tassiopoulos AK, Xue L, Maddox E, Armistead D, Burgess WH and Greisler HP: Heparin-independent mitogenicity
in an endothelial and smooth muscle cell chimeric growth factor (S130K-HBGAM) Amer Jour
Surg ; 188 (5) 575-579, 2004.
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Book Chapters |
| Laredo J, GREISLER HP (2003) Future
Perspective: Tissue engineering? - The World of Mesh Meshes: Benefits and Risks.
Schumpelick V, Nyhus LM, Eds; Springer-Verlag France: Paris. |
| GREISLER HP, (2003) Biomolecules, WTEC
Panel Report of Tissue Engineering Research. Academic Press, San Diego California, 2003. |
| Laredo J, Xue L, GREISLER HP (2004)
Vascular Grafts: Host Interactions. In Encyclopedia of Biomaterials and Biomedical
Engineering, Wuek G, Bowlin G, Eds; Marcel Dekker: New York. (In Press 2004) |
| Laredo J, Xue L, GREISLER HP (2004)
Vascular Grafts: Development Strategies. In Encyclopedia of Biomaterials and Biomedical
Engineering, Wuek G, Bowlin G, Eds; Marcel Dekker: New York. (In Press 2004). |
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